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Fluorescent two-color whole mount in situ hybridization in Platynereis dumerilii (Polychaeta, Annelida), an emerging

Kristin Tessmar-Raible1, Patrick R H Steinmetz, Heidi Snyman

  • 1European Molecular Biology Laboratory, Heidelberg, Germany.

Biotechniques
|October 21, 2005
PubMed
Abstract

No abstract available in PubMed .

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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