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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
High efficiency ethanol fermentation by entrapment of Zymomonas mobilis into LentiKats
M Rebros1, M Rosenberg, R Stloukal
1Faculty of Chemical and Food Technology, Department of Biotechnology, Slovak Technical University, Slovakia. martin.rebros@stuba.sk
Aims:
To examine the potential of Zymomonas mobilis entrapped into polyvinylalcohol (PVA) lens-shaped immobilizates in batch and continuous ethanol production.
Methods And Results:
Cells, free or immobilized in PVA hydrogel-based lens-shaped immobilizates - LentiKats, were cultivated on glucose medium in a 1 l bioreactor. In comparison with free cell cultivation, volumetric productivity of immobilized batch culture was nine times higher (43.6 g l(-1) h(-1)). The continuously operated system did not improve the efficiency (volumetric productivity of the immobilized cells 30.7 g l(-1) h(-1)).
Conclusions:
We demonstrated Z. mobilis capability, entrapped into LentiKats, in the cost-efficient batch system of ethanol production.
Significance And Impact Of The Study:
The results reported here emphasize the potential of bacteria in combination with suitable fermentation technology in industrial scale. The innovation compared with traditional systems is characterized by excellent long-term stability, high volumetric productivity and other technological advantages.
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