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Updated: Aug 15, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Molecular analysis of microbial population transition associated with the start of denitrification in a wastewater
T Hoshino1, T Terahara, S Tsuneda
1Department of Chemical Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan. t-hoshino@ruri.waseda.jp
Aims:
The objective of this study is to determine the bacteria playing an important role in denitrification by monitoring the molecular dynamics accompanying the start of denitrification.
Methods And Results:
cDNA reverse-transcribed from 16S rRNA was amplified with fluorescent labelled primer for terminal restriction fragment length polymorphism (T-RFLP) analysis and an unlabelled primer for cloning analysis. The terminal restriction fragments (T-RFs) that increased in association with the start of denitrification were determined. These T-RFs were identified by in silico analysis of 16S rRNA sequences obtained from cloning. As a result, it was clearly observed that the bacteria belonging to the genera Hydrogenophaga and Acidovorax increased in number after the start of denitrification.
Conclusions:
It was demonstrated that T-RFLP analysis targeting 16S rRNA is appropriate for the daily monitoring of a bacterial community to control wastewater treatment processes. Combination of the results of T-RFLP analysis and 16S rRNA clone library indicated that the bacteria belonging to the genera Hydrogenophaga and Acidovorax play an important role in denitrification.
Significance And Impact Of The Study:
The results of this study provide new insight to the 16S rRNA level of active denitrifying bacteria in wastewater treatment processes.
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