Optimized mRNA isolation and assessment methodology with two-step real time PCR

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Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
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Charoula Psallida1, Dimitris Argyropoulos

  • 1Institute of Biotechnology, National Agricultural Research Foundation, Athens, Greece.

Preparative Biochemistry & Biotechnology
|October 22, 2005
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...