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First nuclear DNA C-values for 18 eudicot families
Lynda Hanson1, Amy Boyd, Margaret A T Johnson
1Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK. l.hanson@kew.org
Annals of Botany
|October 22, 2005
Summary
This study generated new nuclear DNA C-value data for 20 angiosperm families, increasing overall familial representation. Targeted efforts are crucial for meeting plant genome size workshop goals.
Area of Science:
- Plant biology
- Genomics
- Cytogenetics
Background:
- The 2003 Plant Genome Size Workshop aimed to increase angiosperm species DNA C-value data and familial coverage.
- Eudicot families had significantly lower representation (42.5%) compared to monocots (72.8%) and basal angiosperms (69.0%) in 2003.
Purpose of the Study:
- To generate novel nuclear DNA C-value data for underrepresented eudicot families.
- To enhance the overall familial coverage of DNA C-value data in angiosperms.
Main Methods:
- Nuclear DNA C-values were estimated using flow cytometry or Feulgen microdensitometry.
- Chromosome counts were obtained when feasible.
Main Results:
- First nuclear DNA C-values are reported for 20 angiosperm families, including 18 eudicots.
- This research increased angiosperm familial representation to 55.2% and eudicot representation to 48.5%.
Conclusions:
- Targeting specific plant families is vital for improving nuclear DNA C-value representation.
- International collaboration is essential to access unsampled plant families and meet workshop targets.