[Spectrum analysis of erythrocyte intracellular hemoglobin with a novel fast multi-channel micro-spectrophotometry]

With the application of a novel fast multi-channel micro-spectrophotometry (MMSP), the absorption spectrum of intracellular hemoglobin molecule in a human single living red blood cell was monitored under the altered physiological environments (pH, temperature, partial pressure of oxygen, and osmotic pressure). It was found that on the absorption spectrum curve of hemoglobin the spike, the height and the position of the characteristic absorption peaks at 540 and 575 nm were altered obviously with the changes in physiological conditions, indicating that the concentration, structure and function of the hemoglobin in the red blood cell may be closely associated with the physiological environments.