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A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
Published on: August 6, 2013
Generation of biochemical response patterns of different substances using a whole cell assay with multiple signaling
Anke Suska1, Daniel Filippini, Tony P M Andersson
1Division of Applied Physics, Department of Physics and Measurement Technology, Linköping University, SE-581 83, Sweden. anksu@ifm.liu.se
Abstract:
Distinctive generation of biochemical response patterns of eight different substances, using an assay based on pigment containing cells, was demonstrated. Xenopus laevis melanophores, transfected with human beta(2)-adrenergic receptor, were seeded in a 96 well microplate and used to generate individual biochemical images through a two transient measuring protocol that contributes to highlight the response signatures of the agents. Adequate signal processing creates distinctive patterns in a time-concentration response space suitable for substance classification. The concept of biochemical images is introduced here. The assays were evaluated both with a standard microplate reader and with a computer screen photo-assisted technique (CSPT) yielding similar results. Since CSPT platforms only demand standard computer sets and web cameras as measuring setup, applications for these kind of assays outside main-laboratories were discussed.
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