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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
Nucleotide analogues to investigate RNA structure and function
Subha R Das1, Robert Fong, Joseph A Piccirilli
1Howard Hughes Medical Institute, Department of Biochemistry & Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA.
Abstract:
RNA plays an essential cellular role in nearly every aspect of the transmission and expression of genetic information, including regulatory roles that have significance for cellular development. Access to RNA bearing synthetic modifications has allowed biological chemists to probe deep into the inner workings of cellular processes. Here, we describe recent advances in harnessing the power of nucleotide analogues to obtain mechanistic and biological insights into RNA structure, function and dynamics.
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