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Patterning cultured cells by visible light illumination with photosensitizers
Ryoji Waki1, Sumiko Gamo, Masahiko Bessho
1Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Sakai, Osaka 599-8570, Japan.
Journal of Bioscience and Bioengineering
|October 26, 2005
Abstract:
We showed that PC12 cells and 3T3 cells cultured in dishes were killed by illumination with visible white light from a halogen lamp at 7 x 10(4) lx for 5 min in the presence of either 10 microM hematoporphyrin or 2 microM methylene blue as a photosensitizer. This simple technique, based on the photodynamic reaction via generation of reactive oxygen species can be applicable for patterning cultured cells.
