Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for stem cell labeling with fluorine 18.

Bing Ma1, Kurt D Hankenson, James E Dennis

  • 1Department of Radiology, Division of Nuclear Medicine, University of Michigan Medical School, Ann Arbor, 48109, USA.

Nuclear Medicine and Biology
|October 26, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Culture Expansion Alters Human Bone Marrow-Derived Mesenchymal Stem Cell Production of Osteoarthritis-Relevant Cytokines and Growth Factors.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2024
Same author

Multidisciplinary Management Strategies for Long COVID: A Narrative Review.

Cureus·2024
Same author

Amino Acid Uptake Limitations during Human Mesenchymal Stem Cell-Based Chondrogenesis.

Tissue engineering. Part A·2024
Same author

MSCs mediate long-term efficacy in a Crohn's disease model by sustained anti-inflammatory macrophage programming via efferocytosis.

NPJ Regenerative medicine·2024
Same author

The U.S. Food and Drug Administration, the mechanism of action, and other considerations for cell-based therapy candidates.

Experimental biology and medicine (Maywood, N.J.)·2023
Same author

Mesenchymal stem cells ameliorate inflammation in an experimental model of Crohn's disease via the mesentery.

bioRxiv : the preprint server for biology·2023

Hexadecyl-4-[(18)F]fluorobenzoate, a novel radiotracer, was synthesized for labeling mesenchymal stem cells (MSCs). This tracer enables short-term tracking of MSC distribution in vivo, particularly in lung accumulation studies.

Area of Science:

  • Radiochemistry
  • Biomedical Imaging
  • Stem Cell Biology

Background:

  • Mesenchymal stem cells (MSCs) are promising for regenerative medicine but require effective tracking methods.
  • Short-term in vivo distribution studies are crucial for understanding MSC behavior after administration.

Purpose of the Study:

  • To synthesize and characterize Hexadecyl-4-[(18)F]fluorobenzoate ([(18)F]HFB) as a novel radiolabeling agent.
  • To evaluate the utility of [(18)F]HFB for labeling MSCs and tracking their distribution in vivo using microPET imaging.

Main Methods:

  • One-step synthesis of [(18)F]HFB via aromatic nucleophilic substitution.
  • Radiolabeling of rat MSCs by absorption of [(18)F]HFB into cell membranes.
  • MicroPET imaging of intravenously injected, [(18)F]HFB-labeled MSCs in rats.

Related Experiment Videos

Main Results:

  • [(18)F]HFB was synthesized in good yield (52% decay corrected) and high purity (97%).
  • [(18)F]HFB effectively labeled MSCs, with radioactivity absorbed into cell membranes.
  • MicroPET imaging demonstrated high and persistent accumulation of labeled MSCs in the lungs following intravenous injection.

Conclusions:

  • [(18)F]HFB is a readily prepared radiolabeling agent suitable for short-term distribution studies of injected stem cells.
  • The tracer facilitates visualization of MSC localization, particularly in the lungs, using microPET imaging.