Isolation and characterization of TaDof1 transcription factor in wheat (Triticum. aestivum. L)

The Dof (DNA binding with one finger) proteins are plant specific transcription factors. Dof proteins are apparently encoded by a multiple gene family in higher plants. However, only one Dof gene, WPBF, was reported in wheat. In this study, a member of Dof gene family, TaDof1, was cloned from wheat. TaDof1 encode 291 amino acids, with a predicted molecular mass of 30.348 kDa. At its N-terminal end, a 52 amino acid stretch typical of Dof domain and two serine-rich stretches were observed. Sequence alignment indicated that, in Dof domain, TaDof1 share more than 75% identity with other Dof proteins of different species. TaDof1 was expressed highly in leaves and sheaths, but lowly in roots, and constitutively expressed in developing seeds of 2-12 DAP. It was interesting to note that TaDof1 was differentially expressed between hybrids F1 and parents in root, sheath and leaf. The implication of the differential expression patterns of TaDof1 was discussed in related to the up-regulation of C4 pathway related gene in hybrid rice and heterosis.