Related Experiment Video

Updated: Aug 15, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Stable suppression of tumorigenicity by Pin1-targeted RNA interference in prostate cancer

Akihide Ryo1, Hiroji Uemura, Hitoshi Ishiguro

  • 1Department of Pathology, Yokohama City University School of Medicine, Yokohama, Japan. aryo@yokohama-cu.ac.jp

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|October 26, 2005
PubMed
Abstract

Insights

Targeting peptidyl-prolyl isomerase (Pin1) with gene silencing inhibits prostate cancer growth and tumorigenicity. Pin1 depletion suppressed tumor growth, angiogenesis, and enhanced apoptosis, indicating its therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • The peptidyl-prolyl isomerase Pin1 is implicated in oncogenesis across various solid tumors.
  • Pin1's role in prostate cancer progression and tumorigenicity warrants further investigation.

Purpose of the Study:

  • To evaluate the efficacy of Pin1-targeted gene silencing in inhibiting prostate cancer cell growth and tumorigenicity.
  • To explore Pin1 as a potential therapeutic target for prostate cancer, especially recurrent forms.

Main Methods:

  • Retrovirus-mediated RNA interference was employed to silence Pin1 expression in PC3 and LNCaP prostate cancer cell lines.
  • Cellular proliferation, colony formation, migration, invasion, and apoptosis were assessed.
  • Tumorigenic potential was evaluated in vivo using athymic mouse models.

Main Results:

  • Pin1 gene silencing significantly reduced prostate cancer cell proliferation, colony formation, migration, and invasion.
  • Pin1 depletion enhanced apoptosis induced by serum depletion or anticancer agents.
  • In vivo studies demonstrated that Pin1 suppression inhibited tumor growth and angiogenesis in athymic mice.

Conclusions:

  • Pin1 is crucial for both the tumorigenesis and the maintenance of the transformed phenotype in prostate cancer cells.
  • Pin1 represents a promising therapeutic target for prostate cancer, with potential benefits for recurrent tumors.

More Related Videos

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

Published on: May 16, 2012

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...