Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitrate-based vasodilators inhibit multiple vascular aldehyde dehydrogenases.

Tonya C Murphy1, Roberta Arntzen, Matthew J Picklo

  • 1Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota, School of Medicine and Health Sciences, Grand Forks, ND 58203, USA.

Cardiovascular Toxicology
|October 26, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations among Milk Microbiota, Milk Fatty Acids, Milk Glycans, and Inflammation from Lactating Holstein Cows.

Microbiology spectrum·2023
Same author

Time-Restricted Feeding Modifies the Fecal Lipidome and the Gut Microbiota.

Nutrients·2023
Same author

Changes in the Fecal Metabolome Accompany an Increase in Aberrant Crypt Foci in the Colon of C57BL/6 Mice Fed with a High-Fat Diet.

Biomedicines·2022
Same author

Quantifying Sphingomyelin in Dairy through Infusion-Based Shotgun Mass Spectrometry with Lithium-Ion-Induced Fragmentation.

Journal of agricultural and food chemistry·2022
Same author

Alteration in Plasma Metabolome in High-Fat Diet-Fed Monocyte Chemotactic Protein-1 Knockout Mice Bearing Pulmonary Metastases of Lewis Lung Carcinoma.

Nutrition and metabolic insights·2022
Same author

Metabolomes of Lewis lung carcinoma metastases and normal lung tissue from mice fed different diets.

The Journal of nutritional biochemistry·2022

Nitrate-based vasodilators like glyceryl trinitrate (GTN) can inactivate aldehyde dehydrogenase (ALDH) enzymes, potentially affecting atherosclerosis treatment. Different vasodilators show selective ALDH inhibition, impacting detoxification pathways.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Cardiovascular Science

Background:

  • Nitrate-based vasodilators (NBVs) treat atherosclerosis complications.
  • Glyceryl trinitrate (GTN), an NBV, is bioactivated by aldehyde dehydrogenase 2 (ALDH2).
  • ALDH2 detoxifies cytotoxic lipid-aldehydes like HNE, elevated in atherosclerosis.

Purpose of the Study:

  • To test if different NBVs inhibit multiple ALDH enzymes.
  • To investigate the selectivity and mechanisms of NBV-induced ALDH inhibition.

Main Methods:

  • Assessed ALDH2, ALDH3A, and ALDH5A presence and localization in aorta.
  • Measured ALDH enzyme activity inhibition by GTN and isosorbide dinitrate (ISDN).
  • Quantified the effect of GTN on HNE detoxification in rat aorta homogenates.

Related Experiment Videos

Main Results:

  • GTN (1 microM) inhibited ALDH2 (55%) and ALDH3 (ablated) activity.
  • ISDN (1 microM) inhibited ALDH3 (98.9%) but not ALDH2 activity, even at 50 microM.
  • GTN inhibited NAD+-dependent (59%) and NADP+-dependent (75%) HNE detoxification.

Conclusions:

  • GTN and ISDN exhibit selectivity in ALDH enzyme inactivation.
  • The mechanisms of ALDH inactivation by GTN and ISDN differ.
  • NBV-induced ALDH inhibition may impact therapeutic efficacy in atherosclerosis.