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Updated: Aug 15, 2026

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Published on: August 20, 2018
Promoting protein, a silkworm hemolymph protein promoting in vitro replication of nucleopolyhedrovirus, binds to
Seiji Miyake1, Yoshiaki Yamano, Isao Morishima
1Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Japan.
Abstract:
A protein which bound to 125I-labeled peptidoglycan (PGN) was isolated from hemolymph of silkworm larvae. The N-terminal amino acid sequence and the molecular weight of the protein were in accord with those described for Promoting Protein (PP) from the silkworm. The binding of the protein to [125I]PGN was competitively inhibited by various beta-glucans. The binding kinetics of PGN and chitin to the protein were analyzed in a biosensor.
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