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Related Experiment Videos

Immunochemical differences among Methanosarcina mazei S-6 morphologic forms.

R Yao1, A J Macario, E Conway de Macario

  • 1Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany.

Journal of Bacteriology
|July 1, 1992
PubMed
Summary
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Methanosarcina mazei S-6 exhibits distinct growth forms—packets, single cells, and lamina—that interconvert. These morphologic changes occur independently of medium composition, suggesting internal regulatory mechanisms in archaeobacteria.

Area of Science:

  • Microbiology
  • Archaea research
  • Cellular morphology

Background:

  • Methanosarcinae are unique archaeobacteria exhibiting significant morphologic plasticity during growth.
  • Methanosarcina mazei S-6 is the sole known strain displaying three distinct forms: packets, single cells, and lamina.

Purpose of the Study:

  • To investigate the interconversion and distinct characteristics of different growth forms in Methanosarcina mazei S-6.
  • To determine if environmental factors influence the observed morphologic changes.

Main Methods:

  • Culturing of Methanosarcina mazei S-6 under controlled conditions.
  • Analysis of distinct cell forms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
  • Immunoblotting to identify protein differences between morphologic forms.

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Main Results:

  • Two pairs of forms (packets/single cells and single cells/lamina) interconverted within the same growth medium.
  • Distinct biochemical differences were observed between the interconverting forms, as shown by SDS-PAGE and immunoblotting.
  • Morphologic interconversion occurred despite identical medium composition, Ca2+, Mg2+ concentrations, and growth substrate.

Conclusions:

  • Methanosarcina mazei S-6 demonstrates remarkable morphologic plasticity, with distinct forms interconverting under constant environmental conditions.
  • The observed interconversion and biochemical differences suggest intrinsic regulatory mechanisms governing cell differentiation in this archaeal species.
  • Environmental factors are not the sole drivers of morphologic changes in Methanosarcina mazei S-6.