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Updated: Jul 12, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
Published on: November 25, 2015
Genetic organization of a plasmid from an industrial wastewater bioreactor
Michael Bramucci1, Mario Chen, Vasantha Nagarajan
1Central Research and Development, DuPont Company, P.O. Box 80328, Wilmington, DE 19880-0328, USA. Michael.G.Bramucci@usa.dupont.com
Abstract:
Pseudomonas strain CT14 was isolated from activated sludge. Strain CT14 contained a 55, 216 bp plasmid that was characterized by sequence analysis. The plasmid had a modular structure with 51 open reading frames (ORFs) that were distributed between two clearly demarcated domains. Domain I primarily contained genes for plasmid-related functions and a novel origin of replication. Domain II bore evidence of extensive transposition and recombination. Domain II contained several genes from a meta-cleavage pathway for aromatic rings. These genes appeared to have been recruited from different hosts. This observation suggests that sequencing pCT14 may have revealed an intermediate stage in the evolution of a new assemblage of meta-cleavage pathway genes.
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