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Action of UV and visible radiation on chlorophyll fluorescence from dark-adapted grape leaves (Vitis vinifera L.).

Erhard E Pfündel1

  • 1Lehrstuhl für Botanik II, Universität Würzburg, Julius-von-Sachs-Platz 3, D-97082, Würzburg, Germany, pfuendel@botanik.uni-wuerzburg.de.

Photosynthesis Research
|October 26, 2005
PubMed
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Grapevine leaves exposed to natural sunlight showed that while UV radiation impacts photosynthesis, the standard F(V)/F(M) measurement may not fully detect UV-induced damage to photosystem II (PS II). Relative F(M) concentrations better indicated UV inhibition of photosynthesis.

Area of Science:

  • Plant Physiology
  • Photosynthesis Research
  • UV Radiation Effects on Plants

Background:

  • Visible radiation (VIS) and ultraviolet (UV) radiation significantly influence plant physiology.
  • Chlorophyll fluorescence parameters, such as maximum fluorescence (F(M)) and minimum fluorescence (F(0)), are key indicators of photosystem II (PS II) efficiency.
  • Understanding the differential effects of UV-A and UV-B radiation on plant fluorescence is crucial for assessing photosynthetic health.

Purpose of the Study:

  • To investigate the impact of different light conditions, including UV radiation, on grapevine leaf fluorescence.
  • To develop a model describing how natural radiation intensities affect PS II and leaf fluorescence.
  • To determine the reliability of fluorescence measurements in detecting UV-induced PS II inhibition.

Main Methods:

Related Experiment Videos

  • Grapevine plants (Vitis vinifera L. cv. Silvaner and Bacchus) were exposed to controlled light conditions: natural radiation, UV-B screened, and UV-A/UV-B screened.
  • Measurements included maximum chlorophyll fluorescence (F(M)), minimum chlorophyll fluorescence (F(0)), and the F(V)/F(M) ratio.
  • A mathematical model was developed and fitted to experimental data to describe radiation effects on PS II and fluorescence.

Main Results:

  • All light conditions reduced F(M) and increased F(0); increasing UV stimulated F(M) quenching but reduced F(0) increases.
  • UV-B caused a moderate reduction in the F(V)/F(M) ratio, while visible radiation had a clearer effect.
  • The model indicated that UV-inactivated PS II can exhibit relatively high F(V)/F(M), making detection difficult using this ratio alone.

Conclusions:

  • Relative concentrations of intact PS II, derived from F(M), correlated better with CO(2) gas exchange than F(V)/F(M).
  • UV-inactivated PS II is not easily detected by F(V)/F(M) measurements in grapevine leaves.
  • UV inhibition of PS II is a significant factor contributing to the overall inhibition of photosynthesis by natural UV radiation.