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Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation
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Genetics and molecular disorders in severe secondary hyperparathyroidism: lessons from rna and microarray studies

I Santamaria1, D Alvarez-Hernandez, J B Cannata-Andia

  • 1Bone and Mineral Research Unit, Instituto Reina Sofia de Investigacion, Hospital Universitario Central de Asturias, Oviedo - Spain.

Journal of Nephrology
|October 26, 2005
PubMed

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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