Related Experiment Videos
Detection of borreliacidal antibodies in hamsters by using flow cytometry
K F Sachsenmeier1, K Schell, L W Morrissey
1Wisconsin State Laboratory of Hygiene, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison 53706.
Journal of Clinical Microbiology
|June 1, 1992
Abstract:
Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease.