Related Experiment Video
Updated: Aug 15, 2026
Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
Published on: March 3, 2023
[Experimental study of saponins from Tribulus terrestris on renal carcinoma cell line]
Huang-jian Yang1, Wei-jing Qu, Bin Sun
1School of Life Sciences, East China Normal University, Shanghai 200062, China.
Objective:
To investigate the effect of saponins from Tribulus terrestris (STT) on the renal carcinoma cell (786-0) in vitro, and inhibitory mechanisms.
Method:
Effects of SIT on the cytotoxicity, morphological changes of apoptosis, cell cycle and expression of Bcl-2 protein in the 786-0 were tested respectively by MTT method, Wright and acridine orange stain assay, as well as flow cytometry (FCM).
Result:
After the 786-0 was treated by STY, it was shown that: 1) A significant cytotoxic effect was observed by MTT assay; 2) Apoptosis-induced was viewed by Wright and acridine orange stain assay; 3) The distribution of 786-0 on S phase was increased; 4.) The expression of Bcl-2 protein and cyclin D1 was decreased.
Conclusion:
STT can significantly inhibit the growth of 786-0 in vitro, partially, by apoptosis.
