Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Negative feedback in NO/cGMP signalling.

D Koesling1, F Mullershausen, A Lange

  • 1Institut für Pharmakologie und Toxikologie, Medizinische Fakultät, Ruhr-Universität Bochum, Med. Fak. MA N1, Universitätsstrasse 150, 44780 Bochum, Germany. doris.koesling@ruhr-uni-bochum.de

Biochemical Society Transactions
|October 26, 2005
PubMed
Summary

Nitric oxide (NO) signals by activating guanylate cyclase (GC) to increase cyclic GMP (cGMP). Substance YC-1 enhances NO

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of different irrigation water temperatures on the vegetative development of mung beans (Vigna radiata L.).

Brazilian journal of biology = Revista brasleira de biologia·2025
Same author

Advancing technical understanding of the double-seeded gill cell culture system for drug uptake studies in fish.

Environmental toxicology and chemistry·2025
Same author

A blinded randomised split-body clinical trial evaluating the effect of fluorescent light energy on antimicrobial management of canine interdigital furunculosis.

Veterinary dermatology·2025
Same author

Old breeds, new solutions? Effects of two different traditional sire breeds on skin lesions, tail lesions, tail losses, performance and behaviour of rearing pigs.

Animal : an international journal of animal bioscience·2023
Same author

Formation of Re-Aggregated Neonatal Porcine Islet Clusters Improves <i>In Vitro</i> Function and Transplantation Outcome.

Transplant international : official journal of the European Society for Organ Transplantation·2023
Same author

The -2549 -2567 del18 Polymorphism in VEGF and Irreversible Bronchoconstriction in Asthmatics.

Journal of investigational allergology & clinical immunology·2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Nitric oxide (NO) mediates cellular effects primarily through stimulating soluble guanylate cyclase (sGC) and increasing cyclic guanosine monophosphate (cGMP) levels.
  • sGC possesses a heme prosthetic group crucial for NO binding and activation. NO-sensitizing compounds, such as YC-1, enhance sGC activity, presenting potential therapeutic applications.
  • Two sGC isoforms, alpha1beta1 and alpha2beta1, have been identified, differing in tissue distribution and subcellular localization, with alpha2beta1 predominantly found in the brain.

Purpose of the Study:

  • To elucidate the mechanisms of NO-mediated signaling through sGC and cGMP.
  • To investigate the role of NO-sensitizers like YC-1 in modulating sGC activity.
  • To understand the interplay between cGMP synthesis and degradation, particularly the function of phosphodiesterase 5 (PDE5) in NO signaling.

Related Experiment Videos

Main Methods:

  • The study likely involved biochemical assays to measure sGC activity and cGMP production.
  • Experiments may have utilized pharmacological agents like NO donors and YC-1 to probe enzyme sensitization.
  • Cellular studies were likely employed to assess NO-induced cGMP signaling in intact cells, including the investigation of PDE5 activity.

Main Results:

  • NO activates sGC, leading to increased cGMP formation, a key second messenger.
  • Substance YC-1 was identified as an NO-sensitizer, potentiating sGC activity.
  • cGMP itself activates phosphodiesterase 5 (PDE5), which degrades cGMP, establishing a negative feedback loop.
  • This PDE5 activation by cGMP is sustained, contributing to desensitization in NO/cGMP signaling pathways.

Conclusions:

  • NO-induced cGMP signaling is regulated by both synthesis (sGC) and degradation (PDE5).
  • The discovery of NO-sensitizers like YC-1 offers avenues for pharmacological intervention.
  • Sustained PDE5 activation by cGMP plays a critical role in the desensitization of NO/cGMP signaling, impacting cellular responses over time.