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Related Experiment Videos

Nucleolar biogenesis: the first small steps.

J-L Prieto1, B McStay

  • 1Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK.

Biochemical Society Transactions
|October 26, 2005
PubMed
Summary
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The nucleolus, crucial for ribosome production, forms around active ribosomal genes. Upstream-binding factor (UBF) binding to these genes explains their unique chromosomal structure and nucleolar sequestration.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The nucleolus is central to ribosome biogenesis, assembling around ribosomal gene clusters.
  • Nucleolar formation and disassembly are tightly regulated during the cell cycle.
  • Ribosomal gene transcription by RNA polymerase I (Pol I) is key to nucleolar assembly.

Purpose of the Study:

  • To elucidate the role of upstream-binding factor (UBF) in nucleolar organization and function.
  • To explain the cytogenetic features of nucleolar organizer regions (NORs).
  • To understand the sequestration of Pol I transcription machinery within nucleoli during interphase.

Main Methods:

  • Analysis of nucleolar organizer regions (NORs) and their cytogenetic characteristics.
  • Investigation of RNA polymerase I (Pol I) transcription factor interactions.

Related Experiment Videos

  • Studying the binding of UBF to ribosomal gene repeats.

    • Main Results:

    • Extensive UBF binding to ribosomal gene repeats drives the formation of secondary constrictions at NORs.
    • Active ribosomal gene transcription initiates nucleolar assembly.
    • Non-engaged Pol I machinery is sequestered in nucleoli via interaction with UBF.

    Conclusions:

    • UBF binding is essential for the characteristic under-condensation of NORs.
    • Nucleolar structure and function are intrinsically linked to ribosomal gene activity and UBF.
    • The nucleolus acts as a reservoir for transcription machinery through UBF interactions.