Related Experiment Video
Updated: Aug 15, 2026

Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus
Published on: December 16, 2022
The choroid plexus removes beta-amyloid from brain cerebrospinal fluid
Janelle S Crossgrove1, G Jane Li, Wei Zheng
1School of Health Sciences, Purdue University, 550 Stadium Mall Drive, CIVL-1163D, West Lafayette, IN 47907, USA.
The choroid plexus may reduce beta-amyloid (Abeta) in cerebrospinal fluid (CSF). This brain barrier shows selective Abeta uptake and clearance, potentially benefiting Alzheimer's disease patients.
Area of Science:
- Neuroscience
- Biochemistry
- Cell Biology
Background:
- The choroid plexus acts as a barrier between blood and cerebrospinal fluid (CSF).
- Beta-amyloid (Abeta) accumulation in the brain is linked to Alzheimer's disease.
- Understanding Abeta regulation in CSF is crucial for neurodegenerative disease research.
Purpose of the Study:
- To investigate the role of the choroid plexus in regulating beta-amyloid (Abeta) concentration in the CSF.
- To determine the mechanisms of Abeta uptake and transport by the choroid plexus.
Main Methods:
- Isolated rat choroid plexus tissue was used to measure Abeta uptake (volume of distribution, V(D)).
- Radioactive tracers ([125I]Abeta1-40 and [14C]sucrose) were employed to quantify uptake and extracellular space.
- Primary cultures of choroidal epithelial cells in Transwells assessed Abeta permeability and cellular accumulation.
Main Results:
- Abeta uptake by the choroid plexus was time-dependent, temperature-dependent, and saturable.
- Transthyretin and apolipoprotein E3 did not affect Abeta uptake.
- Choroidal epithelial cells demonstrated greater Abeta permeability and cellular accumulation from the CSF-facing side, indicating efflux bias.
Conclusions:
- The choroid plexus actively and selectively clears beta-amyloid (Abeta) from the cerebrospinal fluid (CSF).
- This clearance mechanism may involve an undetermined efflux process from the CSF.
- The choroid plexus's role in Abeta regulation could be a therapeutic target for Alzheimer's disease.
More Related Videos
09:58A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side
Published on: May 6, 2016
06:19Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease
Published on: October 20, 2023
Related Concept Videos
Cerebral Edema ll: Pathophysiology
The Blood-brain Barrier
Cerebral Edema l: Introduction
Cerebrospinal Fluid
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain.
Cytotoxic Edema: Pathophysiology
Brain Abscess l: Introduction