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Updated: Aug 15, 2026

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
Published on: July 17, 2019
Oxidation of biotin during oligonucleotide synthesis
Krishna Upadhya1, Iqbal K Khattak, Bashar Mullah
1Applied Biosystems, 8a50 Lincoln Centre Drive, Foster City, CA 94404, USA. upadhykg@appliedbiosystems.com
Abstract:
A new "polystyrene biotin support" has been synthesized for the solid support synthesis of the 3'-biotinylated oligonucleotides. Several oligos were synthesized and were analyzed by the HPLC and Mass Spec. Oligo analysis revealed that the biotin gets oxidized to "biotin sulfoxide" during the synthesis.
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