Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA recognition by fluor-aromatic substituted.

A Zivković1, J W Engels

  • 1Institute for Organic Chemistry and Chemical Biology, Johann-Wolfgang-Goethe University, Frankfurt aM., Germany.

Nucleosides, Nucleotides & Nucleic Acids
|October 27, 2005
PubMed
Summary

Researchers synthesized novel fluorinated RNA building blocks to expand the study of RNA structure and function. These modified nucleosides offer new possibilities for investigating RNA base stacking, pairing, and hydrogen bonding interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulation of IL-33/ST2-TIR and TLR signalling pathway by fingolimod and analogues in immune cells.

Scandinavian journal of immunology·2014
Same author

Photo-physical properties of 2-(1-ethynylpyrene)-adenosine: influence of hydrogen bonding on excited state properties.

Physical chemistry chemical physics : PCCP·2014
Same author

Microdialysate analysis of monoamine neurotransmitters--a versatile and sensitive LC-MS/MS method.

Analytica chimica acta·2013
Same author

Molecular pharmacological profile of a novel thiazolinone-based direct and selective 5-lipoxygenase inhibitor.

British journal of pharmacology·2011
Same author

A New Experiment for the Measurement of nJ(C,P) Coupling Constants Including 3J(C4'i,Pi) and 3J(C4'i,Pi+1) in Oligonucleotides.

Journal of biomolecular NMR·2010
Same author

[Agenda 2010 - competitive and knowledge-based : XVI International Heidelberg Anesthesia Symposium, 27-28th March 2010].

Der Anaesthesist·2010

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry

Background:

  • Ribonucleic acid (RNA) possesses significant structural diversity, crucial for biological functions.
  • Natural RNA is limited to four canonical bases (A, U, C, G), restricting investigations into its structural parameters.
  • Exploring modified bases can enhance understanding of RNA's structural and functional repertoire.

Purpose of the Study:

  • To synthesize a diverse library of fluoromodified RNA building blocks.
  • To incorporate these novel building blocks into an RNA sequence to study their impact.
  • To investigate the effects of these modifications on RNA structural properties using biophysical methods.

Main Methods:

  • Chemical synthesis of various fluorinated phenyl and benzimidazole-based RNA amidites.

Related Experiment Videos

  • Oligonucleotide synthesis incorporating a single modified base at a defined position within an A, U-rich sequence.
  • UV melting experiments to analyze the thermal stability and structural integrity of modified RNA duplexes.

    • Main Results:

    • Successful synthesis of multiple fluoromodified RNA building blocks, including phenyl and benzimidazole derivatives with varying degrees of fluorination.
    • Incorporation of these modified amidites into a 12-mer RNA sequence.
    • UV melting data provided insights into how different fluoromodifications affect RNA duplex stability.

    Conclusions:

    • The synthesized fluoromodified RNA building blocks offer a versatile toolkit for probing RNA structure-function relationships.
    • These modifications can modulate RNA duplex stability, providing avenues for designing novel RNA-based molecules.
    • Further studies can elucidate the specific impact of each modification on RNA secondary and tertiary structures.