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Updated: Aug 15, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Prosit, an online service to calculate pseudorotational parameters of nucleosides and nucleotides
Guangyu Sun1, Johannes H Voigt, Victor E Marquez
1Laboratory of Medicinal Chemistry, NCI-Frederick, CCR, NCI, NIH, DHHS, Frederick, Maryland, USA.
Abstract:
The online service PROSIT (Pseudo-Rotational Online Service and Interactive Tool) is a free service available at http://cactus.nci.nci.gov/prosit/ that performs pseudorotational analysis of nucleosides(tides). PROSIT reads the 3D coordinates of nucleosides and returns the pseudorotational phase angle P, puckering amplitude vmax and other related information. As examples, the sugar conformations in a parallel-stranded guanine tetraplex and a four-way Holliday junction are presented here.
