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Updated: Aug 15, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
A new approach to oligonucleotide N3'-->P5' phosphoramidate building blocks
Daria Zielinska1, Krisztina Pongracz, Sergei Gryaznov
1Geron Corporation, Menlo Park, California, USA.
Abstract:
A new synthetic approach to 5-phosphoramidites of 3'-aminonucleosides was developed. The methodology relies upon the use of 3'-amino-2',3'-dideoxy nucleosides as the key starting materials. The final phosphoramidite products were obtained with high yields via 2-3-step efficient chemical transformations using selective introduction of orthogonal protective groups to the 3'-aminonucleoside sugar and base moieties.
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