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Updated: Aug 15, 2026

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
Published on: December 28, 2016
Novel way of capping mRNA trimer and studies of its interaction with human nuclear cap-binding complex
Remigiusz Worch1, Janusz Stepinski, Anna Niedzwiecka
1Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland.
Abstract:
Binding of mRNA 5' cap by the nuclear cap-binding complex (CBC) is crucial for a wide variety of mRNA metabolic events. The interaction involving the CBP20 subunit of CBC is mediated by numerous hydrogen bonds and by stacking of the tyrosine sidechains with two first bases of the capped mRNA. To examine a possible role of a longer mRNA chain in the CBC-cap recognition, we have synthesized an mRNA tetramer using a novel way of capping an RNA trimer and determined its affinity for CBC by fluorescence titration.
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