Related Experiment Video
Updated: Aug 15, 2026
Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues
Published on: July 8, 2020
[Experimental study on induction of the normal human melanocytes apoptosis in vitro by cycloheximide and TNF-alpha]
Zhuang-Qun Yang1, Peng Li, Jun-Bo Tu
1Department of Oral and Maxillofacial Plastic Surgery, Stomatological College, Xi'an Jiao Tong University, Xi'an 710004, China.
Objective:
To investigate the effects of cycloheximide and TNF-alpha on melanocyte (MC).
Methods:
Melanocyte apoptosis was studied with MTT, transmitting electron microscopy and fluorescence labeling of alive cells.
Results:
We added TNF-alpha and cycloheximide in melanocytes, and the typical apoptosis appeared 24 hours later, with chromatin condensation, nuclear pyknosis and apoptotic bodies formation. The results of cytometry showed the typical apoptotic peak.
Conclusion:
TNF-alpha and cycloheximide together could inhibit MC proliferation and induce MC apoptosis.
