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Updated: May 5, 2026

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test
Published on: September 3, 2015
Ultrasonic songs of male mice
Timothy E Holy1, Zhongsheng Guo
1Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri, USA. holy@wustl.edu
Abstract:
Previously it was shown that male mice, when they encounter female mice or their pheromones, emit ultrasonic vocalizations with frequencies ranging over 30-110 kHz. Here, we show that these vocalizations have the characteristics of song, consisting of several different syllable types, whose temporal sequencing includes the utterance of repeated phrases. Individual males produce songs with characteristic syllabic and temporal structure. This study provides a quantitative initial description of male mouse songs, and opens the possibility of studying song production and perception in an established genetic model organism.

