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The effect of G-CSF in an experimental MRSA graft infection in mice
Canan Agalar1, Erol Eroglu, Mustafa Sari
1Department of Infectious Diseases and Clinical Microbiology, Kirikkale University, School of Medicine, Kirikkale, Turkey.
Abstract:
Wound infection after prosthetic material implantation is a troublesome complication with an incidence of 2% to 10%. The effect of granulocyte colony-stimulating factor (G-CSF) was studied in an experimental methicillin-resistant Staphylococcus aureus (MRSA) graft infection model. Eighty adult mice were used. Under general anesthesia an abdominal incision of 2 cm in length was performed. A subcutaneous cavity of 2 x 2 cm in size was created. Polypropylene mesh pieces of 2 x 1 cm and MRSA solution of 0.1ml of 10(8) CFU/mL were used. G-CSF was applied systemically or locally in a dosage of 0.02 MU/30 g body weight. There were 8 groups: group I, wound + MRSA; group II, wound + mesh + MRSA; group III, wound + mesh + MRSA + G-CSF (ip, 48 h before operation); group IV, wound + mesh + MRSA + G-CSF (ip, 24 h before operation); group V, wound + mesh + MRSA + G-CSF (locally, into the cavity); group VI, wound + mesh (incubated in G-CSF solution for 4 h) + MRSA; group VII, wound + mesh + MRSA + G-CSF, ip, 24 h from operation; and group VIII (positive control group), wound + mesh + MRSA + Teicoplanin (0.03 mg/30 g body weight, ip, 1/2 h before operation). Three days after, animals were killed and incisions were examined for possible infection or abscess formation and wound failure. Meshes were removed; after vortexing and dilution, samples were incubated with 5% agar media. Results of bacterial incubation were evaluated 24 h and 48 h later. There were symptoms of wound infection and abscess formation in all groups except group VIII. In group VIII, MRSA was isolated in 7 events with a colony count below 10(3). Bacterial counts were above 10(6) (10(6)-10(8)) in all other groups. Thus, it was observed that wound infection could be created with this model, but G-CSF could not prevent the development of wound infection, whether it was administered systemically or locally. Teicoplanin decreased the number of colony-forming units of MRSA, and prevents wound infection in this MRSA wound infection model.
Insights
Granulocyte colony-stimulating factor (G-CSF) did not prevent methicillin-resistant Staphylococcus aureus (MRSA) wound infections in a mouse model. Teicoplanin, however, effectively reduced MRSA colony counts and prevented infection.
Area of Science:
- Surgical Infections
- Biomaterials Science
- Infectious Diseases
Background:
- Wound infection post-prosthetic material implantation occurs in 2-10% of cases.
- Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in these infections.
- Granulocyte colony-stimulating factor (G-CSF) is investigated for its potential therapeutic effects.
Purpose of the Study:
- To evaluate the efficacy of G-CSF in preventing MRSA graft infections in a murine model.
- To compare systemic and local G-CSF administration.
- To assess the effectiveness of G-CSF against Teicoplanin, a known antibiotic.
Main Methods:
- An experimental MRSA graft infection model was established in 80 adult mice using polypropylene mesh.
- G-CSF was administered systemically or locally at various time points.
- Infection was assessed by examining incisions for abscess formation and quantifying bacterial load on meshes.
Main Results:
- All groups treated with G-CSF (systemic or local) showed symptoms of wound infection and abscess formation.
- Bacterial counts in G-CSF treated groups ranged from 10^6 to 10^8 CFU/mL.
- The positive control group treated with Teicoplanin showed significantly reduced MRSA counts (<10^3 CFU/mL) and no infection.
Conclusions:
- G-CSF, administered systemically or locally, failed to prevent MRSA wound infection in this experimental model.
- Teicoplanin demonstrated efficacy in preventing MRSA wound infection and reducing bacterial load.
- This model successfully established MRSA wound infections, but G-CSF was not an effective preventative agent.
