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Related Experiment Videos

Seasonally hibernating phenotype assessed through transcript screening.

Daryl R Williams1, L Elaine Epperson, Weizhong Li

  • 1School of Biological Sciences, University of Liverpool, United Kingdom.

Physiological Genomics
|October 27, 2005
PubMed
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Hibernation involves significant gene expression changes, particularly in heart and brain tissues, with a shift towards lipid metabolism and reduced urea cycle activity. The RNA-binding protein RBM3 is a key winter-induced transcript across tissues.

Area of Science:

  • * Physiology and molecular biology of mammalian hibernation.
  • * Comparative genomics and transcriptomics.
  • * Energy metabolism and cellular adaptation.

Background:

  • * Hibernation is a complex physiological adaptation for winter energy conservation.
  • * Molecular mechanisms underlying hibernation transitions remain incompletely understood.
  • * Previous studies have offered selective insights into hibernation's genetic basis.

Purpose of the Study:

  • * To conduct a large-scale gene expression screen in golden-mantled ground squirrels (Spermophilus lateralis).
  • * To identify transcriptional responses during summer-winter and torpor-arousal transitions.
  • * To elucidate tissue-specific molecular adaptations during hibernation.

Main Methods:

  • * Utilized 112 cDNA microarrays with 12,288 probes, covering over 5,109 genes.

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  • * Analyzed gene expression profiles in liver, cardiac, and brain tissues.
  • * Employed Gene Ontology profiling for functional interpretation of transcriptomic data.

    • Main Results:

    • * Liver showed modest winter downregulation (102 cDNAs), primarily related to detoxification and urea cycle.
    • * Cardiac tissue exhibited distinct changes in torpor and arousal (59-115 cDNAs upregulated).
    • * Brain displayed upregulated transcripts (78 cDNAs), with downregulated electron transport; RBM3 was universally upregulated.

    Conclusions:

    • * Hibernation involves modest, tissue-specific transcriptional reprogramming.
    • * Identified a shift towards lipid beta-oxidation in liver and heart during winter.
    • * Highlighted RBM3 as a conserved, winter-induced transcript across tissues, crucial for hibernation phenotype.