Related Experiment Video
Updated: Aug 15, 2026

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
Published on: June 28, 2016
[Resolution of carotenoid isomers in Lycium barbarum L. by heuristic evolving latent projection]
1College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. lu_hongmei225@126.com
Abstract:
Lycium barbarum L., a kind of traditional Chinese herb, is found to have bioactivities such as anticancer, antioxidant, hypoglycemic and immunological activities. In both in vitro and in vivo studies, the carotenoids were found to be a class of the effective compounds. The carotenlysoids in Lycium barbarum L. were separated by high perforance liquid chromatography with diode array detection (HPLC-DAD). Seven peaks were obtained by HPLC on a C18 column with acetonitrile-methylene chloride (60:40, v/v) as mobile phase at a flow-rate of 1.0 mL/min. Most of the peaks that had been validated as single peaks in the 2-dimensional chromatography were found to be overlapping peaks. The overlapping chromatographic peaks were resolved by chemometric method--Heuristic Evolving Latent Projection (HELP) based on 3-dimensional data. As an example, the chromatogram and UV spectra of 4 isomers were obtained by resolving an overlapping peak. These results showed that the combination of chemometric methods and modern analytical instruments provides an effective method for the analysis of complex systems such as isomers.
Related Concept Videos
Racemic Mixtures and the Resolution of Enantiomers
Stereoisomerism of Cyclic Compounds
Stereoisomers
Prochirality
Fischer Projections
Stereoisomerism
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
