Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

E3 ubiquitin ligases.

Helen C Ardley1, Philip A Robinson

  • 1Molecular Medicine Unit, University of Leeds, Clinical Sciences Building, St. James's University Hospital, Leeds LS9 7TF, UK. h.c.ardley@leeds.ac.uk

Essays in Biochemistry
|October 28, 2005
PubMed
Summary

Ubiquitin-protein ligases (E3s) are crucial for targeting proteins for degradation via the ubiquitin-26 S proteasome system (UPS). Their diverse structures and functions ensure precise protein clearance, vital for cellular health and preventing disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Potential Sources of Stress in Students at a UK Veterinary School Prior to Abattoir Visits.

Journal of veterinary medical education·2025
Same author

Efficacy and safety of acalabrutinib with best supportive care versus best supportive care in patients with COVID-19 requiring hospitalization.

ImmunoHorizons·2025
Same author

Identifying veterinary surgeons' barriers to, and potential solutions for, improving antimicrobial stewardship among sheep farmers in Northern Ireland.

Veterinary record open·2024
Same author

Reducing Hospitalizations and Multidrug-Resistant Organisms via Regional Decolonization in Hospitals and Nursing Homes.

JAMA·2024
Same author

Antibiotic use in the Northern Irish sheep flock: What lessons can be learnt from medicine records and farmer attitudes to improve stewardship of these essential medicines?

Preventive veterinary medicine·2024
Same author

SVEPM 2023, the annual conference of the Society for Veterinary Epidemiology and Preventive Medicine: Toulouse finally welcomes us in person.

Preventive veterinary medicine·2024

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • The ubiquitin-proteasome system (UPS) regulates protein degradation.
  • Ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s) mediate substrate selectivity in the UPS.
  • E3 ligases are diverse, possessing HECT, RING, or U-box domains, and can act alone or in complexes.

Purpose of the Study:

  • To elucidate the critical role of E3 ligases in substrate recognition and protein degradation.
  • To highlight the structural diversity and functional mechanisms of E3 ligases.
  • To underscore the importance of E3 ligases in cellular processes and disease.

Main Methods:

  • Analysis of protein-protein interactions within the UPS.
  • Characterization of E3 ligase domains (HECT, RING, U-box).
  • Review of E3 ligase involvement in multi-protein complexes.

Main Results:

  • E3 ligases, in conjunction with E2 enzymes, confer specificity to the UPS.
  • Post-translational modifications can precede substrate selection by E3s.
  • RING and U-box E3s function as adaptors, bringing E2 enzymes and substrates together.

Conclusions:

  • E3 ligases are essential for precise, spatio-temporal protein clearance in eukaryotes.
  • Dysregulation of E3 ligase function is linked to various diseases.
  • E3 ligases represent a powerful mechanism for cellular protein homeostasis.

Related Experiment Videos