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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Multiplex PCR for simultaneous detection of macrolide and tetracycline resistance determinants in streptococci
Surbhi Malhotra-Kumar1, Christine Lammens, Jasper Piessens
1Belgian Reference Center for Group A Streptococcus, University of Antwerp, Belgium. surbhi.malhotra@ua.ac.be
Abstract:
Resistance to macrolides and tetracyclines is increasing among streptococci and co-occurs as their resistance determinants are carried on the same mobile element. We developed a multiplex PCR to facilitate simultaneous and specific detection of resistance determinants for both macrolides [erm(A), erm(B), and mef(A/E)] and tetracyclines [tet(M), tet(O), tet(K), and tet(L)] in streptococci.