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A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia.

George Adrian Calin1, Manuela Ferracin, Amelia Cimmino

  • 1Department of Molecular Virology, Immunology, and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA.

The New England Journal of Medicine
|October 28, 2005

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View abstract on PubMed

Summary

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  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • A Microrna Signature Associated With Prognosis And Progression In Chronic Lymphocytic Leukemia.
    • This summary is machine-generated.

    A unique microRNA signature identifies chronic lymphocytic leukemia (CLL) prognostic factors and disease progression. Mutations in microRNA transcripts are common in CLL and may impact disease.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • MicroRNA (miRNA) expression profiles can differentiate normal B cells from malignant B cells in chronic lymphocytic leukemia (CLL).
    • Prognostic factors in CLL are crucial for treatment decisions and patient outcomes.
    • Investigating miRNA profiles in relation to prognostic factors may reveal new insights into CLL pathogenesis.

    Purpose of the Study:

    • To determine if microRNA (miRNA) expression profiles are associated with known prognostic factors in chronic lymphocytic leukemia (CLL).
    • To identify specific miRNA signatures linked to disease progression and patient outcomes in CLL.

    Main Methods:

    • MicroRNA expression profiles were evaluated in 94 CLL samples.
    • Expression levels of ZAP-70, IgV(H) gene mutational status, and time to initial treatment were assessed.
    • Genomic sequences of 42 miRNA genes were analyzed for abnormalities.

    Main Results:

    • A distinct 13-gene miRNA signature differentiated CLL cases based on ZAP-70 expression and IgV(H) mutational status.
    • This miRNA signature correlated with disease progression.
    • Germ-line mutations in the miR-16-1-miR-15a precursor were identified, leading to reduced miRNA expression and associated with allele deletion.

    Conclusions:

    • A specific microRNA signature is linked to prognostic factors and disease progression in chronic lymphocytic leukemia (CLL).
    • Mutations within microRNA transcripts are frequently observed in CLL patients and may play a functional role in the disease.

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