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[Enhancement of wound healing by taspine and its effect on fibroblast]
Yalin Dong1, Langchong He, Fang Chen
1School of medicine, Xi' an Jiaotong University, Xi' an 71006.
Objective:
To study the effect of taspine on enhancement of skin wound healing and its effect on fibroblast proliferation and autocrine.
Methods:
The plerosis effect of taspine on experimental skin wound was observed in vivo. Different concentrations of taspine were added in vitro and MTT technique was applied to observe its effect on fibroblast proliferation, the levels of transforming growth factor-beta1 (TGF-13P) and epidermal growth factor (EGF) were determined by ELISA.
Results:
In vivo, exo-applied taspine 300 microg and 150 microg accelerated the recovery of skin wound. In vitro, 0.50-0.4 microg/ml taspine could increase autocrine of TGF-beta1and EGF by fibroblast, but it showed no effect on L929 fibroblast proliferation.
Conclusions:
Taspine enhances wound healing by increasing the autocrine of TGF-beta1 and EGF by fibroblast.
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