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Related Experiment Videos

NG2 expression regulates vascular morphology and function in human brain tumours.

C Brekke1, A Lundervold, P Ø Enger

  • 1Department of Biomedicine, Section for Anatomy and cell biology, University of Bergen Jonas Lies Vei 91, N-5009 Bergen, Norway.

Neuroimage
|October 29, 2005
PubMed
Summary

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NG2 expression on glioma cells enhances tumour angiogenesis and vascular leakiness. This proteoglycan influences tumour progression by altering blood vessel structure and function, highlighting its role in tumour-host interactions.

Area of Science:

  • Neuro-oncology
  • Cancer biology
  • Vascular biology

Background:

  • Tumour angiogenesis is crucial for cancer growth, involving complex tumour-host interactions.
  • The mechanisms regulating these interactions are not fully understood.
  • NG2 proteoglycan expression on tumour cells can promote proliferation and angiogenesis.

Purpose of the Study:

  • To investigate the role of NG2 expression in glioma tumour growth and progression.
  • To assess the impact of NG2 on tumour vascularization and host tissue response.

Main Methods:

  • Engineered NG2-positive (U251-NG2) and NG2-negative (U251-WT) human glioma cell lines.
  • Implanted cells into immunodeficient rat brains and monitored tumours using multispectral MRI with two contrast agents.

Related Experiment Videos

  • Performed immunohistochemistry and electron microscopy to analyze tumour vasculature.

    • Main Results:

    • NG2-expressing tumours showed increased vascular leakiness, particularly for larger contrast agents.
    • U251-NG2 tumours exhibited significantly higher vasogenic oedema and microvascular density compared to controls.
    • Vessels in NG2-expressing tumours had larger gaps between endothelial cells.

    Conclusions:

    • Tumour cell NG2 expression can modulate the function and structure of host-derived tumour vasculature.
    • NG2 plays a significant role in the communication between tumour cells and the host environment.
    • Targeting NG2 may offer a strategy to inhibit tumour angiogenesis and progression.