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Updated: Aug 15, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Low-density lipoprotein cholesterol determines oxidative stress and endothelial dysfunction in saphenous veins from

Sammy Al-Benna1, Carlene A Hamilton, John D McClure

  • 1BHF Glasgow Cardiovascular Research Centre, Division of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.

Arteriosclerosis, Thrombosis, and Vascular Biology
|October 29, 2005
PubMed

Insights

Oxidative stress and superoxide levels are elevated in coronary artery disease (CAD) patients, contributing to endothelial dysfunction. Lowering low-density lipoprotein (LDL) cholesterol is crucial for managing this condition.

Area of Science:

  • Cardiovascular Science
  • Oxidative Stress Research
  • Endothelial Function Studies

Background:

  • Endothelial dysfunction is linked to cardiovascular disease.
  • The specific role of oxidative stress in this relationship requires further elucidation.

Purpose of the Study:

  • To investigate the role of oxidative stress in endothelial dysfunction in patients with severe coronary artery disease (CAD).
  • To determine the impact of low-density lipoprotein (LDL) cholesterol on endothelial function and oxidative stress markers in CAD patients.

Main Methods:

  • Studied 188 severe CAD patients and 51 healthy controls.
  • Assessed saphenous vein relaxation, vascular superoxide levels, and oxidative stress markers.
  • Utilized calcium ionophore, apocynin, and allopurinol for relaxation studies.

Main Results:

  • CAD patients exhibited significantly decreased saphenous vein relaxation compared to controls (26% vs 60%).
  • Vascular superoxide production was markedly increased in CAD patients (0.89 vs 0.56 nmol/mg per min).
  • Apocynin and allopurinol showed a greater effect on vasorelaxation in CAD patients, indicating increased oxidative stress.

Conclusions:

  • Endothelial dysfunction in CAD is associated with elevated superoxide and oxidative stress biomarkers in saphenous veins.
  • LDL cholesterol is a primary determinant of endothelial dysfunction and oxidative stress in these patients.
  • Findings support aggressive LDL cholesterol-lowering therapies for CAD management.
Abstract

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