Quantitative analysis of platelet-activating factor in rat brain
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A Tokumura1, T Yotsumoto, T Hoshikawa
1Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Life Sciences
|January 1, 1992
Abstract:
Age-related decrease of the platelet-activating factor (PAF) content in rat brain was shown by a convenient method consisting of solid extraction of lipids with a Sep-Pak C-18 cartridge, lipid separation by HPLC and bioassay on rabbit platelets. This method was sufficiently sensitive to allow measurement of PAF in a single brain, and the recovery of PAF was quite high throughout the procedure.