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Microarray immunoassay for phenoxybenzoic acid using polymer encapsulated Eu:Gd2O3 nanoparticles as fluorescent

Mikaela Nichkova1, Dosi Dosev, Shirley J Gee

  • 1Department of Entomology, University of California Davis, Davis, California 95616, USA.

Analytical Chemistry
|November 1, 2005
PubMed
Summary

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Europium-doped gadolinium oxide nanoparticles offer a photostable alternative to organic dyes for microarray bioanalysis. These novel fluorescent probes enable sensitive detection of biomarkers like phenoxybenzoic acid in immunoassay microarrays.

Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Traditional microarray bioanalysis relies on organic dyes, which suffer from photobleaching and spectral overlap issues.
  • Developing stable, photoluminescent probes is crucial for advancing biosensing technologies.

Purpose of the Study:

  • To investigate the use of crystalline europium-doped gadolinium oxide (Eu:Gd2O3) nanoparticles as fluorescent labels in immunoassay microarrays.
  • To overcome the limitations of organic dyes in bioanalytical detection.

Main Methods:

  • Synthesized Eu:Gd2O3 nanoparticles via spray pyrolysis and functionalized them with poly(L-lysine) (PL).
  • Confirmed PL shell formation using TEM, colloidal stability studies, and amino group quantification.
  • Conjugated PL-encapsulated nanoparticles to antibodies for a competitive fluorescence microimmunoassay.

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Main Results:

  • Eu:Gd2O3 nanoparticles exhibited narrow red emission, large Stokes shift, and long fluorescence lifetime (1 ms).
  • Successfully applied functionalized nanoparticles as reporters in a microimmunoassay for phenoxybenzoic acid (PBA).
  • Achieved a limit of detection of 1.4 µg/L for PBA using confocal fluorescence microscopy and internal standard calibration.

Conclusions:

  • Lanthanide oxide nanoparticles show potential as fluorescent probes for microarray and biosensor technology.
  • This approach offers a stable and sensitive alternative for immunodiagnostics and high-throughput screening.
  • Eu:Gd2O3 nanoparticles represent a promising advancement in fluorescent labeling for bioanalysis.