Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Design for DNA separation medium using bacterial cellulose fibrils.

Mari Tabuchi1, Yoshinobu Baba

  • 1Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Science, The University of Tokushima COE, 1-78 Shomachi, Tokushima 770-8505, Japan. tabuchi@ph.tokushima-u.ac.jp

Analytical Chemistry
|November 1, 2005
PubMed
Summary

Researchers developed a new bacterial cellulose medium for high-resolution DNA separation. This novel material effectively separates small DNA fragments and single-nucleotide polymorphisms using electrophoresis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Routine monitoring of microRNAs in salivary exosomes using a cellulose nanofiber sheet.

Biosensors & bioelectronics·2026
Same author

Quantum Life Science: A Paradigm for Life Science Research.

ACS nano·2025
Same author

Enhancing Near-Infrared Photoluminescence of Ag<sub>8</sub>GeS<sub>6</sub> Quantum Dots Through Compositional Fine-Tuning and ZnS Coating for In Vivo Bioimaging.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

A multiplex microfluidic device to detect miRNAs for diagnosis of plant growth status.

Horticulture research·2025
Same author

Nanobiodevices and quantum life science for future healthcare.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025
Same author

Quantum life science: biological nano quantum sensors, quantum technology-based hyperpolarized MRI/NMR, quantum biology, and quantum biotechnology.

Chemical Society reviews·2025

Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Bacterial cellulose (BC) possesses a unique 3D nanofibrous network structure.
  • Existing DNA separation media face limitations in resolving small DNA fragments and single-nucleotide polymorphisms.
  • Developing advanced materials is crucial for improving DNA analysis resolution.

Purpose of the Study:

  • To introduce a novel DNA separation medium utilizing bacterial cellulose fibrils.
  • To demonstrate the efficacy of this medium for high-resolution electrophoretic DNA separation.
  • To characterize the structural properties of the bacterial cellulose medium.

Main Methods:

  • Incorporation of bacterial cellulose fibrils into a low-viscosity polymer solution (<5 cP).

Related Experiment Videos

  • Fabrication of a double-mesh separation medium with a 10-nm flexible mesh and 10-nm to 1-microm rigid pores from BC fragments.
  • Electrophoretic separation of DNA fragments, including 10-100 bp and single-nucleotide polymorphism (SNP) analysis.

    • Main Results:

    • The bacterial cellulose medium achieved high-resolution electrophoretic separation of DNA.
    • Effective separation of small DNA fragments (10-100 bp) was demonstrated.
    • The medium showed promise for distinguishing single-nucleotide polymorphisms.

    Conclusions:

    • Bacterial cellulose fibrils can be utilized to create advanced media for high-resolution DNA separation.
    • The novel double-mesh structure enhances separation efficiency for small DNA fragments and SNPs.
    • This BC-based medium offers a promising alternative for sensitive DNA analysis.