Related Experiment Video
Updated: Aug 14, 2026
Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
Published on: February 3, 2016
[Dynamic observation of lung metastasis model of adenoid cystic carcinoma]
Shao-wei Chen1, Hong-zhang Huang, Xi-qiang Liu
1Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Sun Yet-sen University, Guangzhou 510055, China.
Objective:
To form ACC-M-GFP cells by transfecting pEGFP-1 into ACC-M cells, and to build up lung metastasis model of adenoid cystic carcinoma for dynamic observation in simulated lung environment.
Methods:
pEGFP-1 was prepared and then transfected into ACC-M cell lines by using cationic lipid-based gene transfer technique. After successive selection, the ACC-M-GFP cells were collected and inoculated in BALB/C mice. The simulated lung environment was built up, and dynamic observation was performed by laser confocal microscopy within four weeks after transfection.
Results:
The amount of fluorescent tumor cell colonies and the intensity of fluorescence gradually increased from the second week to the forth week after transfection. In the meantime, only a very small number of tumor cells had the ability to form clones.
Conclusions:
We successfully built up a lung metastasis model of adenoid cystic carcinoma for dynamic observation. The model is suitable for capturing and analyzing metastatic tumor cells in early stage.
