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[Enhanced prokaryotic expression of a recombinant Lagurus lagurus zona pellucida subunit 3 with codon optimization]
Yi-jie Li1, Fu-chun Zhang, Yu Zhang
1Key Laboratory of Molecular Biology, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China. jaylixj@hotmail.com
Aim:
To improve prokaryotic expression level of Lagurus lagurus zona pellucida 3(LZP3) gene.
Methods:
The rare codons of LZP3 gene fragment were turned into the most frequently used codons in E. coli by overlap PCR. LZP3 gene and mutated LZP3 (LZP3m) gene were cloned into expression vector pGEX4T-1 respectively, and transformed into E. coli BL21(DE3).
Results:
The expression level of LZP3m was notably higher than that of LZP3.
Conclusion:
The expression of LZP3 gene in E. coli could be successfully improved by using codon optimization.
