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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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The direct genetic encoding of pyrrolysine

Joseph A Krzycki1

  • 1Department of Microbiology, OSU Biochemistry Program, Ohio State University, Columbus, OH 43210, USA. krzycki.1@osu.edu

Current Opinion in Microbiology
|November 1, 2005
PubMed
Summary

Pyrrolysine, a non-canonical amino acid, is encoded by the amber codon (UAG) in Methanosarcinaceae. Its genetic code incorporation involves a unique pyrrolysyl-tRNA synthetase and tRNA, similar to the original 20 amino acids.

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