Related Experiment Video
Updated: Aug 15, 2026
07:41
Transdermal Measurement of Glomerular Filtration Rate in Mechanically Ventilated Piglets
Published on: September 13, 2022
Intestinal microdialysis--applicability, reproducibility and local tissue response in a pig model
K J Emmertsen1, P Wara, F B Soerensen
1Clinical Institute, Aarhus University Hospital, Skejby Hospital, Aarhus N, Denmark. kje@studmed.au.dk
Summary
Intestinal wall microdialysis shows significant inflammation, limiting its use for monitoring ischemia. Future research should explore the intra-peritoneal approach for more reliable metabolic measurements.
Area of Science:
- Gastroenterology
- Surgical Research
- Biomedical Engineering
Background:
- Microdialysis is a technique used to monitor local tissue ischemia.
- Its application to the intestinal wall requires further investigation for reliability.
Purpose of the Study:
- To assess the applicability and reproducibility of microdialysis in the intestinal wall.
- To evaluate the local response to microdialysis probes in the intestinal wall.
- To compare intestinal wall microdialysis with intra-peritoneal and muscle placements.
Main Methods:
- Microdialysis probes were inserted into the ileal wall, peritoneal cavity, and psoas muscle of 12 pigs.
- Relative recovery was determined using the no net flux method.
- Metabolite levels (glucose, lactate, glycerol) were measured over six hours, followed by histological examination.
Main Results:
- Significant variations in relative recovery were observed across all probe locations.
- No significant changes in absolute metabolite values were detected during the study.
- A severe inflammatory response was noted in the ileal wall surrounding the probes.
- Lactate levels in the blood were 25-30% of intra-tissue values.
Conclusions:
- Accurate metabolite measurements via microdialysis necessitate the measurement of relative recovery.
- Inflammation in the intestinal wall likely impacts metabolic measurements.
- Intestinal wall microdialysis is currently best suited for experimental research.
- The intra-peritoneal approach may offer a more viable alternative for future studies.
