Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ginkgolides induce apoptosis and decrease cell numbers in mouse blastocysts.

Wen-Hsiung Chan1

  • 1Department of Bioscience Technology, Center for Nanotechnology, Chung Yuan Christian University, Chung Li, Taiwan. whchan@cycu.edu.tw

Biochemical and Biophysical Research Communications
|November 2, 2005
PubMed
Summary

Ginkgolides from Ginkgo biloba extracts induce apoptosis and reduce cell numbers in mouse blastocysts. This leads to impaired early embryonic development and increased early-stage blastocyst death in vitro.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PM<sub>2.5</sub>-modulated targets and miRNAs associated with lung cancer and injury are protected by natural products.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Co-culture with adipose mesenchymal stem cells promotes Blastocyst formation and gene expression in embryos from aged mice.

Regenerative therapy·2025
Same author

Effects and regulatory mechanisms of bisphenol a on the increases apoptosis and decreases differentiation potential in mouse embryonic stem cells.

Toxicology research·2025
Same author

Long-Term Safety Evaluation of Fluorescent Gold Nanoclusters Conjugated with α-Lipoic Acid: Insights from a Six-Month In Vivo Study.

Journal of functional biomaterials·2025
Same author

Fluorescent gold nanoclusters possess multiple actions against atherosclerosis.

Redox biology·2024
Same author

Dose-dependent effects of silver nanoparticles on cell death modes in mouse blastocysts induced via endoplasmic reticulum stress and mitochondrial apoptosis.

Toxicology research·2024

Area of Science:

  • Reproductive biology
  • Developmental toxicology
  • Pharmacology

Background:

  • Ginkgo biloba extracts are widely used, but their effects on early embryonic development are not fully understood.
  • Ginkgolides are major active components of Ginkgo biloba extracts.
  • Understanding the impact of ginkgolides on preimplantation embryos is crucial for assessing reproductive safety.

Purpose of the Study:

  • To investigate the cytotoxic effects of ginkgolides A and B on mouse blastocysts.
  • To evaluate the impact of ginkgolides on early postimplantation embryonic development in vitro.
  • To determine the mechanisms underlying ginkgolide-induced embryonic toxicity.

Main Methods:

  • Mouse blastocysts were treated with varying concentrations of ginkgolide A or B.

Related Experiment Videos

  • Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was used to detect apoptosis.
  • Cell counts of total cells, trophectoderm, and inner cell mass were quantified.
  • In vitro implantation and subsequent embryonic development were monitored.

    • Main Results:

    • Ginkgolide A and B treatment significantly increased apoptosis in blastocysts.
    • Ginkgolide exposure led to a reduction in total cell numbers and lineage-specific cell counts.
    • While initial implantation rates were unaffected, treated embryos showed significantly reduced development to later stages.
    • Embryos treated with ginkgolides exhibited increased early-stage death.

    Conclusions:

    • Ginkgolides induce apoptosis and decrease cell proliferation in mouse blastocysts.
    • Ginkgolide treatment retards early postimplantation development and increases embryonic lethality.
    • These findings highlight potential risks associated with Ginkgo biloba extract consumption during early pregnancy.