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Updated: Aug 12, 2026
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
[Experimental study on the inhibitory effect of Carboxymethyl Pachymaram on hepatitis B virus expression from
Hui-ping Dhan1, An-ji Hou, Fu-er Lu
1Department of Combination of Chinese Medicine and Western Medicine of Zhongnan Hospital, Wuhan University, Wuhan 430070, China.
Objective:
To observe the anti-HBV activity of Carboxymethyl Pachymaram (CMP) on the culturing of 2.2.15 cell line.
Methods:
Concentrations of 20.0 g/L, 12.0 g/L, 6.0 g/L, 3.0 g/L, 1.5 g/L of CMP were used to evaluate its toxicity to the cell line and the inhibition rates of the secretion of HBsAg and HBeAg in the cultured 2.2.15 cell line.
Results:
Experiments showed that the mean half toxicity concentration of CMP for 2.2.15 cell line was 13.6 g/L and concentration for 50% inhibition of the secretion of HBsAg and HBeAg were 4.45 g/L, 5.61 g/L and TI were 3.06 and 2.42. CMP showed stronger effect on anti-HBV than aciclovir.
Conclusion:
CMP has good inhibitory effect on the secretion of HBsAg and HBeAg on cultured cell line 2.2.15.
