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Related Experiment Videos

CO uptake kinetics of red cells and CO diffusing capacity.

R B Reeves1, H K Park

  • 1Department of Physiology, School of Medicine, State University of New York, Buffalo 14214.

Respiration Physiology
|April 1, 1992
PubMed
Summary

Carbon monoxide (CO) uptake in red blood cells is reaction-limited in normal and high oxygen conditions, challenging the Bohr-Krogh assumption. This study quantifies CO uptake rates for improved understanding of gas exchange.

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Area of Science:

  • Physiology
  • Biophysics
  • Biochemistry

Background:

  • Understanding carbon monoxide (CO) uptake by hemoglobin is crucial for respiratory physiology.
  • Previous models often simplified diffusion barriers and reaction kinetics within red blood cells.
  • The Bohr-Krogh assumption regarding intracellular CO partial pressure during uptake requires re-evaluation.

Purpose of the Study:

  • To accurately measure the rate of CO displacing oxygen from hemoglobin in intact red blood cells.
  • To compare experimental CO uptake rates with theoretical predictions using the Gibson-Roughton equation.
  • To determine new values for the specific conductance of whole blood for CO.

Main Methods:

  • Spectrophotometric measurement of CO uptake in whole blood thin films to minimize diffusion barriers.

Related Experiment Videos

  • Controlled step-changes in CO tension against various background oxygen (O2) tensions.
  • Comparison of initial CO uptake rates with calculations based on the Gibson-Roughton rate equation.

    • Main Results:

    • Measured CO uptake rates in red cells quantitatively aligned with the theoretical Gibson-Roughton rate equation for PO2 > 100 Torr.
    • New values for theta CO (specific conductance of whole blood) were determined under varying CO and O2 tensions.
    • The study found that intracellular CO partial pressure is not zero during CO uptake, refuting the Bohr-Krogh assumption.

    Conclusions:

    • Red blood cell CO uptake is primarily limited by reaction kinetics under normoxic and hyperoxic conditions.
    • Pulmonary capillary CO diffusion equilibrium is achieved rapidly.
    • The Bohr-Krogh assumption that red cell PCO equals 0 during CO uptake is inaccurate, necessitating revised physiological models.