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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Epidemiological aspects of rotavirus infection in Ahwaz, Iran
Alireza Samarbafzadeh1, Elham Mazaheri Tehrani, Manouchehr Makvandi
1Department of Virology, Ahwaz University of Medical Sciences, Ahwaz, Iran.
Insights
Rotavirus infection is common in children in Ahwaz, Iran, affecting 29.5% of those studied. The highest rates were observed in children aged 7-12 months, indicating a significant age-related risk for rotavirus gastroenteritis.
Area of Science:
- Virology
- Pediatrics
- Public Health
Background:
- Rotavirus is a leading cause of severe diarrhea in children globally.
- Understanding regional rotavirus prevalence is crucial for public health interventions.
Purpose of the Study:
- To determine the prevalence and characteristics of rotavirus infections in children in Ahwaz, Iran.
- To analyze the relationship between age and rotavirus infection rates.
Main Methods:
- Stool samples were collected from 200 children (aged 1-24 months) in Ahwaz.
- Polyacrylamide gel electrophoresis (PAGE) was used for rotavirus isolation and characterization.
- Samples were analyzed from both inpatient and outpatient settings.
Main Results:
- Rotavirus was detected in 29.5% of the children studied.
- Infection rates were higher in inpatients (36.5%) compared to outpatients (26.3%).
- The highest prevalence was observed in children aged 7-12 months (statistically significant, p < 0.05).
- The long (L) electrophoretype (78%) was predominant, followed by the short (S) electrophoretype (20.3%).
Conclusions:
- Rotavirus infection is a significant cause of childhood diarrhea in Ahwaz, Iran.
- Age is a critical factor in rotavirus infection rates among young children.
- Further epidemiological studies across Iran are needed to inform prevention strategies.
Abstract:
Rotavirus is the major cause of diarrhoea in children worldwide. In this study, conducted in the city of Ahwaz, Iran, during November 2001-March 2002, stool samples from 200 inpatient (n=63) and outpatient (n=137) children aged 1-24 month(s) were analyzed. Polyacrylamide gel electrophoresis was used for isolating rotavirus. Rotavirus was isolated from 36 (26.3%) of the 137 stool samples of outpatients and from 23 (36.5%) of the 63 stool samples of inpatients. The overall frequency of rotavirus in this population was 29.5%. The highest detection of rotavirus was made in children aged 7-12 months, which demonstrated that the relationship between age and rate of rotaviral infection was statistically significant (p < 0.05). The predominant electrophoretic pattern detected was the long (L) electrophoretype (46 of 59; 78%), followed by the short (S) electrophoretype (12 of 59; 20.3%). One strain had a mixed pattern. Such analysis throughout Iran would assist in developing sound guidelines for the prevention of rotavirus infections.
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